Journal: Cancer Medicine

Article Title: An antibody‐drug conjugate targeting a GSTA glycosite‐signature epitope of MUC1 expressed by non‐small cell lung cancer

doi: 10.1002/cam4.3554

Figure Lengend Snippet: Staining of non‐small cell lung cancer (NSCLC) cells and tissues by 16A (specific to GSTA neoantigen), SM3, and C595 (specific to PDTR neoantigen). (A) NSCLC cell lines were stained with 16A, SM3, and C595 antibodies. (B) Tissue array slides containing consecutive tissue sections from the same NSCLC patients were stained with 16A, SM3, and C595 antibodies.

Article Snippet: Tumor tissue array slides were obtained from CrownBio.

Techniques: Staining

Journal: Cancer Medicine

Article Title: An antibody‐drug conjugate targeting a GSTA glycosite‐signature epitope of MUC1 expressed by non‐small cell lung cancer

doi: 10.1002/cam4.3554

Figure Lengend Snippet: Expression of the aberrantly glycosylated MUC1 peptide motif in lung and breast cancer tissues upon 16A antibody staining. (A) H‐score of the cancer tissue array stained with the 16A antibody. The intensity of IHC staining was scored at four levels: 0 (negative), 1 (weak staining), 2 (medium staining), and 3 (strong staining). The percentages of tumor cells at different intensity levels were evaluated. Total Score = (% at 0) ×0 + (% at 1) × 1 + (% at 2) ×2 + (% at 3) × 3. (B) 16A positivity in lung cancer, breast cancer, and triple‐negative breast cancer (TNBC) samples. (C, D, E, F) Representative photographs of MUC1 immunostaining in peritumoral (C), lung cancer (D), Non‐TNBC (E), and TNBC (F) tissue with the 16A antibody (original magnification ×200). Positivity was defined as ≥30% of tumor with staining ≥2+.

Article Snippet: Tumor tissue array slides were obtained from CrownBio.

Techniques: Expressing, Staining, Immunohistochemistry, Immunostaining

Journal: Glycobiology

Article Title: A strategy for generating cancer-specific monoclonal antibodies to aberrant O -glycoproteins: identification of a novel dysadherin-Tn antibody

doi: 10.1093/glycob/cwz004

Figure Lengend Snippet: Immunohistochemistry with mAb 6C5. Tissue microarrays of breast cancer tissues (A–D), ovarian cancer (E), stomach cancer (F), and the corresponding tumor adjacent normal tissue (G–I) stained with mAb 6C5. MAb 6C5 showed cell surface immunofluorescence in four types of breast cancers, i.e. carcinoma simplex (A), infiltrating duct carcinoma (B), scirrhous carcinoma (C), atypical medullary carcinoma (D) as well as serous papillary cystadenocarcinoma from ovary (E) and adenocarcinoma from stomach (F). Cancer adjacent normal breast (G) and ovary tissue (H) did not react with mAb 6C5, whereas cancer adjacent normal appearing stomach tissue presented with strong intracellular granular staining in most mucous producing cells (left pointing arrow) and a few of those also gave a more homogenous pattern throughout the cell (right pointing arrow) (I).

Article Snippet: For immunohistochemistry, formalin fixed paraffin embedded tissue microarrays (Biomax) or were heated at 60°C 30 min, dewaxed, rehydrated and subjected to antigen retrieval by microwave treatment at pH 6 (citrate buffer).

Techniques: Immunohistochemistry, Staining, Immunofluorescence